acr sc 51504 santa cruz  (Santa Cruz Biotechnology)

Journal: International Journal of Molecular Sciences

Article Title: Effect of IL-1β on the Development of Spermatogenesis In Vitro in Normal and Busulfan-Treated Immature Mice

doi: 10.3390/ijms25094926

Figure Lengend Snippet: Histology of testes from normal and busulfan-treated mice and identification of developed cells in the MCS. Testes from immature mice (7-day-old) (normal mice; A ) or from busulfan-injected mice (10 days post injection of the immature mice) (BU-treated mice; B ) were fixed in Bouin’s solution and stained with hematoxylin–eosin for histological examination. Cells were enzymatically isolated from the testes of mice described above and were cultured for 5 weeks in methylcellulose 3D culture (MCS) containing StemPro Medium, various growth factors, and KSR 10% (see in the absence (CT) or presence of IL-1β (1, 10, and 100 pg/mL) (IL-1β). The morphologies of cultures (from normal and BU-treated mice) in the absence (CT) or presence of IL-1β (IL-1β) after 5 weeks show the presence of cells and organoids ( B ). Developed cells from normal ( C ) and busulfan-treated mice ( D ) were collected after 5 weeks, fixed, and stained with specific antibodies to markers specific for the different stages of spermatogenesis, such as the pre-meiotic marker (Vasa and GFRα), meiotic marker (Boule), and post-meiotic marker (acrosin). DAPI (blue) was used for nucleus staining (NC, negative control; BC, before culture; CT, control (without IL-1β)). Cultures were grown in the presence of different concentrations of IL-1β (1, 10, 100 pg/mL) (IL-1β). The number of repeated experiments was (N) = 4–5, and the number of wells for each treatment in each experiment was ( n ) = 3. We present pictures representative of the experiments.

Article Snippet: Primary antibodies: polyclonal Vasa rabbit anti-mouse (1:200, NBP2-24558; Novus Biologicals), mouse monoclonal Boule (1:100, sc-166660, Santa Cruz Biotechnology, Santa Cruz, CA, USA), polyclonal acrosin rabbit anti-mouse (1:100, NBP-14260, Novus Biologicals).

Techniques: Injection, Staining, Isolation, Cell Culture, Marker, Negative Control, Control

Journal: International Journal of Molecular Sciences

Article Title: Effect of IL-1β on the Development of Spermatogenesis In Vitro in Normal and Busulfan-Treated Immature Mice

doi: 10.3390/ijms25094926

Figure Lengend Snippet: Quantification of the effect of IL-1β on the percentages of developed spermatogenic cells and the expression levels of their markers in cultures from normal mice. Testicular cells were isolated from seminiferous tubules of normal immature mice and cultured in an MCS (see ) in the absence or presence of IL-1β (1, 10, and 100 pg/mL). Cultured cells were collected after 5 weeks and stained with Vasa ( A2 ), Boule ( B2 ), and acrosin ( C2 ). In addition, RNA expression levels of Vasa ( A1 ), Boule ( B1 ), and acrosin ( C1 ) were examined in these cultures by qPCR analysis using specific primers for each factor. GAPDH was used as a housekeeping gene. CT, control (without IL-1β); BC, before culture. The number of repeated experiments was (N) = 10, and the number of wells for each treatment in each experiment was (n) = 8. * compared to CT. # compared to BC. p -values: * p < 0.05, ** p < 0.01, *** p < 0.0001, ## p < 0.01, ### p < 0.001. $ p <0.05, $$$ p < 0.001.

Article Snippet: Primary antibodies: polyclonal Vasa rabbit anti-mouse (1:200, NBP2-24558; Novus Biologicals), mouse monoclonal Boule (1:100, sc-166660, Santa Cruz Biotechnology, Santa Cruz, CA, USA), polyclonal acrosin rabbit anti-mouse (1:100, NBP-14260, Novus Biologicals).

Techniques: Expressing, Isolation, Cell Culture, Staining, RNA Expression, Control

Journal: International Journal of Molecular Sciences

Article Title: Effect of IL-1β on the Development of Spermatogenesis In Vitro in Normal and Busulfan-Treated Immature Mice

doi: 10.3390/ijms25094926

Figure Lengend Snippet: Quantification of the effect of IL-1β on the percentages of developed spermatogenic cells and the expression levels of their markers in cultures from busulfan-treated mice. Testicular cells were enzymatically isolated from seminiferous tubules of busulfan-treated immature mice and cultured in an MCS as described in . Cultured cells were collected after 5 weeks and stained with Vasa ( A2 ), Boule ( B2 ), and acrosin ( C2 ). In addition, RNA expression levels of Vasa ( A1 ), Boule ( B1 ), and acrosin ( C1 ) were examined in these cultures through qPCR analysis using specific primers for each factor. GAPDH was used as a housekeeping gene. CT, control (without IL-1β); BC, before culture. The number of repeated experiments was (N) = 4, and the number of wells for each treatment in each experiment was (n) = 8. * compared to CT. # compared to BC. p -values: ** p < 0.01, *** p < 0.0001, ### p < 0.001.

Article Snippet: Primary antibodies: polyclonal Vasa rabbit anti-mouse (1:200, NBP2-24558; Novus Biologicals), mouse monoclonal Boule (1:100, sc-166660, Santa Cruz Biotechnology, Santa Cruz, CA, USA), polyclonal acrosin rabbit anti-mouse (1:100, NBP-14260, Novus Biologicals).

Techniques: Expressing, Isolation, Cell Culture, Staining, RNA Expression, Control

Journal: International Journal of Molecular Sciences

Article Title: Effect of IL-1β on the Development of Spermatogenesis In Vitro in Normal and Busulfan-Treated Immature Mice

doi: 10.3390/ijms25094926

Figure Lengend Snippet: Summary of the effect of IL-1β on the percentages of spermatogenic cells developed in vitro from cultures of cells isolated from seminiferous tubules of normal and busulfan-treated immature mice.

Article Snippet: Primary antibodies: polyclonal Vasa rabbit anti-mouse (1:200, NBP2-24558; Novus Biologicals), mouse monoclonal Boule (1:100, sc-166660, Santa Cruz Biotechnology, Santa Cruz, CA, USA), polyclonal acrosin rabbit anti-mouse (1:100, NBP-14260, Novus Biologicals).

Techniques: In Vitro, Isolation

Journal: Nanomaterials

Article Title: Oxidative Stress and Acrosomal Status of Human Spermatozoa Subjected to Hydrophobic Carbon Soot Treatments

doi: 10.3390/nano14050395

Figure Lengend Snippet: Mean value and standard deviation (StD) of the ( a ) optical density and ( b ) acrosin content of soot-free (control) and soot-treated semen after Human Acrosin ELISA Kit assays. Both figures reveal similar tendencies, but the difference between control and soot-treated samples in b is more pronounced due to a cumulative effect, since the OD-acrosin dependence is logarithmic (see ). The large StD in control specimens reflects the inherent diversity of native human ejaculates of all patients involved in the study (see ).

Article Snippet: MIOXSYS analytical device and Human Acrosin ELISA Kit were received from Caerus Biotech (Nyon, Switzerland) and MyBioSource (San Diego, SC, USA) respectively.

Techniques: Standard Deviation, Control, Enzyme-linked Immunosorbent Assay